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Are satellite colonies sensitive to ampicillin?

Answer:
Yes, satellite colonies may be observed radiating from the edges of large, well-established colonies. These satellite colonies are not ampicillin-resistant, but grow in an "antibiotic shadow", where ampicillin in the media has been broken down by the large resistant colony. Satellite colonies are generally a sign of antibiotic weakened by not cooling medium enough before adding antibiotic, long-term storage of more than 30 days, or overincubation.
First answer by ID1365986162. Last edit by ID1365986162. Question popularity: 1 [recommend question].

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