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Resolving power = 0.5x wavelength/ numerical aperture (n sin theta)

n sin theta in most microscope have value = 1.2 and 1.4

therefore:

R. P. = 0.5x500nm/ 1.25 = 200nm = 0.2 microns.

(conv. 1000nm = 1micron).

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Q: Calculate the limit of resolution for the oil lens of your microscope Assume an average wavelength of 500nm?
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