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You could but this is not a very practicable way to run an air pump. There will have to be a large amount of batteries in series and parallel connections to add up to the amp hour capacity to run the air pump. Just use an old 12 volt car battery for your project.

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Q: Can You join disposable or rechargeable batteries together to run a 12v air pump for a live bait tank that is meant to plug into a car plug?
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