MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
A minimal amount of normal gram positive intestinal flora can theoretically grow on a Macconkey agar. However, none are known to have actually grown on it.
No. MacConkey agar is used to grow gram negative bacilli
S. epidermidis is gram positive cocci
no bile salts inhibit all sp of streptococci except enterococci
No, MacConkey agar is selective for Gram negative bacteria. Bacillus subtilis is a Gram positive bacterium. So there should be little to no growth on the MacConkey agar.
I don't think so, because the bile salts in McConkey agar will kill it. but I suppose Streptococcus agalactie could.
yes. since streptococcus is gram positive though it will not grow on MacConkey's or SSA (salmonella-shigella agar)
no because it is gram positive and MacConkey agar does not allow growth of most gram positive bacteria due to presence of crystal violet
this MacConkey with crystal violet can grow enterococcus
E.coli shows pink colour colonies in MacConkey agar due to fermentation of lactose
You probably got a false negative. M. luteus should be oxidase +. To determine M. luteus do a MSA plate which should not produce acid and barley grow. That means the plate will look red with a streak of yellow colonies due to the fact that M. luteus produces a yellow pigment.
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
Yes. But because it is not a lactose fermenter, the colonies will be colorless (or sometimes have a green tint).
Yes micrococcus luteus, along with micrococcus roseus both grow on MSA. But, they do not fermente on this agar giving a negative test. However, Staphylococcus aureus grows on MSA and fermentes giving a positive test. *Side note* MSA plate is used to test for G+ coccus. The plate contains salt and salt "loving" bacteria will grow and show yellow colony, example of S. aureus.
MacConkey agar
this MacConkey with crystal violet can grow enterococcus
yes
The organism would be expected to grow better in nutrient agar. Nutrient agar is rich in nutrients and microorganisms grow well when there is availability of nutrients.
E.coli shows pink colour colonies in MacConkey agar due to fermentation of lactose
campylobacter can growth in mcconkey agar.
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
You probably got a false negative. M. luteus should be oxidase +. To determine M. luteus do a MSA plate which should not produce acid and barley grow. That means the plate will look red with a streak of yellow colonies due to the fact that M. luteus produces a yellow pigment.
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
Staphylococcus will not grow on Mac since crystal violet inhibits the growth of Gram positivesfmchinea: Actually some species of staphylococcus grow on MacConkey's plate including Staphylococcus aureus.
Yes. But because it is not a lactose fermenter, the colonies will be colorless (or sometimes have a green tint).