around neutral pH the bacteria carry an overall negative charge in its surface. since the acid dyes have a chromophore ( color giving organic part of the stain) with a negative charge, it will not stain the bacteria.
Bacteria is stained to identify the organism and its structure. Staining also can help in identification of a species. Bacteria are translucent in appearance therefore a stain is applied to allow...
Stains are made of chemicals with loose ends. These ends stick to loose ends on what they stain.
IN ADDITION:.
A stain is a pigment that has to have a surface that will accept it. Meaning that if a...
gram staining is a biochemical method of identifying bacteria in a more specific way.Thus it is important to differentiate gram positive and gram negative bacteria.
The purpose of staining is: 1) - stains make microbes stand out against the background. 2) - helps investigators group major categories 3) - helps to distinguish parts of a cell.