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Difference between spectrophotometry and differential spectrophotometry?

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Answer:

differential spectrophotometry is a spectrophotometric analytical technique in which a solution of the sample's major component is placed in the reference cell and the recorded spectrum represents the difference between the sample cell and the reference cell...basically it uses major component of system as reference and NOT solvent ..for example if a enzyme ligand system is to be assayed ..enzyme + solvent is reference and enzyme + ligand + solvent is test sample..its for quantitative detection i think..

First answer by Anuttamakulkarni. Last edit by Anuttamakulkarni. Contributor trust: 7 [recommend contributor ]. Question popularity: 8 [recommend question].

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