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Sodium chloride help to separate DNA from other proteins.
Dahlia Miller
Wiki User
∙ 12y agoCellular and histone proteins bound to the DNA can be removed either by adding a protease or by having precipitated the proteins with sodium or ammonium acetate, or extracted them with a phenol-chloroform mixture prior to the DNA-precipitation.
If desired, the DNA can be resolubilized in a slightly alkaline buffer or in ultra-pure water. Generally, the acetate-compound strength is high to keep up with the alkaline environment for keeping the DNA solubilised.
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Why you add sodium acetate in dna extraction?
you add sodium acetate to neutralize the charge on the sugar-phosphate backbone of DNA
What is the function of ammonium acetate in DNA extraction?
ammonium acetae use to percipitate DNA from water.
How sodium acetate precipitate DNA?
The sodium acetate disrupts the solvent shell created by the water; which is what makes DNA soluble in water. So as you could imagine, if the solvent shell is disrupted the DNA precipitates out.
Role of sodium chloride in DNA extraction?
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA.
What does Sodium Lauryl Sulfate do in DNA extraction?
sds is an anionic detergent; close relative of sodium lauryl sulphate.it helps in cell disruption by acting on the cell membrane lipids and proteins
Why you add sodium acetate in dna extraction?
you add sodium acetate to neutralize the charge on the sugar-phosphate backbone of DNA
Sodium acetate in plant DNA extraction?
yes
What is the function of ammonium acetate in DNA extraction?
ammonium acetae use to percipitate DNA from water.
What are the function of sodium acetate in dna isolation?
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
What does sodium citrate do in DNA extraction?
how to make sodium citrate in 10% ethanol for DNA extraction
Are there alternatives of potassium acetate in DNA extraction?
to take out the detergents
How sodium acetate precipitate DNA?
The sodium acetate disrupts the solvent shell created by the water; which is what makes DNA soluble in water. So as you could imagine, if the solvent shell is disrupted the DNA precipitates out.
What is the function of 2-propanol in DNA extraction?
To achieve precipitation DNA.
Function TE in extraction DNA?
chelating Mg2+
What is the function of dithiothreitol in DNA extraction?
DTT is the reducing agent for thiolated DNA
What is the function of chloroform in DNA extraction?
It solubalize lipids and protiens to remove them from DNA
