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Osmotic shock is rapid change in solute concentration outside cell.If sucrose is provided to cell as hypotonic environment, the cell allows water to move in and swell resulting in bursting of cell.

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13y ago
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14y ago

The first few steps in DNA isolation are based on getting the DNA out of the cell. This means the cell has to be broken and the cytoplasmic contents released. After which, the nucleus has to be broken to release the DNA.

The function of the lysis buffer is to aid in the breaking of the cell membrane (or cell wall in plants). The lysis buffer contains protease enzymes and essential salts to bring about this process.

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13y ago

It provides Osmotic Shock to the Blood cells ( leukocytes). By adding sucrose to the solution, it absorbs (osmosis) glucose from the cell, hence cell is shrinked and ruptured. so nucleus is available for extraction.

(By Nawaz Naji)

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Q: What is the function of sucrose in DNA extraction?
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