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The relation between A and T is:

A = -log T = - log (I / Io).

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Q: What is the relationship between absorbance and transmittance?
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What is the difference between absorbance and transmittance?

Transmittance is the ratio of light energy that is falling on a body. Absorbance is how much light energy is actually going into the body.


How can you figure out Absorbance without percent transmittance?

A=logIo/I


What happens to results when the cuvette is not correctly placed?

You get no light transmittance or absorbance.


What is the advantage of reading the absorbance rather than the percent transmittance on the spectrophotometer?

because, aliens


Why don't you plot transmittance vs wavelength?

Sometimes you do in fact. In IR spectroscopy, the transmittance is commonly plotted instead of absorbance.However, in most types of spectroscopy, the absorbance is plotted instead. This is due to Beer's Law, which states that the absorbance is directly proportional to the concentration of the sample, the absorptivity of the sample, and the path length (in contrast, the transmittance is exponentially related to those things). Because of the much simpler direct proportionality, absorbance is preferred in many cases.


Why is absorbance not measured directly?

Simply because we cannot measure light absorbed. We are, however, able to measure light transmitted through the use of a spectrophotometer. The device works by shining light of a specific wavelength on a substance and measuring the amount of light that gets through. This "transmittance" has a negative logarithmic relationship to absorbance.


Why do too densely coloured samples fail to give proper results using spectrophotometer?

Because we measure transmittance in spectrophotometer and for densely coloured samples transmittance will be very less. So we will not get proper results for absorbance.


What is external calibration and is it the same as the manual calibration of equipment?

Well, external calibration is a method used in analytical chemistry to determine the concentration of an unknown analyte. In essence, you take known concentrations of the analyte and plot it on an absorbance or transmittance graph to get a linear plot. And then you take that linear equation and plug in the absorbance or transmittance value received from the unknown solution and get the concentration. An example of this is if you want to find out the amount of calcium in a vitamin tablet. Dissolve the vitamin tablet and test the solution to get an absorbance value. Then test by the same method various concentrations of a calcium solution, plot this on a graph of absorbance vs. concentration and there yah go.


What happens to light that strikes clear plastic?

A common technique in chemistry is spectroscopic analysis. This is where light is passed through a solution to determine its composition. The spectrometer will give a reading of absorbance or percent transmittance. The absorbance tells you how much light is absorbed, while the percent transmittance tells you what percentage of the light passed through and was not absorbed. If when you say clear you mean "colorless," all the light will pass through. The plastic is most likely not entirely colorless so only a small portion of the light will be absorbed, and it will have a high percent transmittance value.


Why do chemists prefer to read the absorbance rather than the percent transmittance of lght when analyzing a sample having a visible color?

Because when you graph a molar concentration vs. absorbance graph, the graph is linear, making the graph easier to read.


What is effect of solvent in uv spectroscopy?

effect of solvent on UV-Visible spectrum


Why do you zero the spectrophotometer on 0 and 100 transmittance and not absorbancy?

None of the physical materials in the world are perfectly transparent. Even when light passes through air, some part of it gets scattered due to dust particles on its way. however, when one is interested in knowing the transparency of a material ( solid/liquid), such losses can be held constant and the photometer can be calibrated to estimate the trasmittance(transparency) by selecting the endpoints. for example, if a piece of thick black India rubber is held between a source of light and the detector, one can set the output to read zero transmittance and after removing it to 100 % transmittance. If any material is now held between the two, the output will show a change in transmittance that truly responds to only the sample and no other interfering inputs. With absorbance, this may not be true since in certain cases, the loss attributed to absorbance might in fact be due to other mechanisms such as scaterring or (regular)reflection.