How can you isolate a gene with the help of transposon?

By restriction enzymes or a process called gel electrophoresis.

To be frank, transposons effect genes when they move, or jump to a different location and cause mutations. If it doesn't cause a mutation, it inactivates the gene. For instance, in Indian Corn, some...

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Every few cell division, transposons will copy it self and simply pick up and move else where, jumping at random to a new location on the chromosomes taking the genes with it.