Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
A common design for estimating the concentrations of compounds in biological samples is the serial dilution assay, in which measurements are taken at several different dilutions of a sample, giving several opportunities for an accurate measurement. Curren tly, serial dilution is a standard tool in the fields of toxicology and immunology.
Serial dilution helps to choose a dilution which is relevant to our experiment.
Often the standard which is given to you in the lab is far to strong for the experiment and it needs to be diluted. But equally the equipment has a detection limit so we can't dilute it to much, or if it is too diluted the experiment might not work.
Its a technique used to reduce the number of colonies that could grow in a culture. 1ml of the sample specimen is diluted into 99ml of saline then 1ml of that solution is put into a 2nd tube that has 99ml of saline and finally 1ml of the 2nd solution in transferred into a 3rd test tube with 99ml of saline.
The first dilution (the one with the original sample) is diluted 1/100 the second 1/10,000 and the 3rd one by 1/1,000,000
use C1V1=C2V2
1:2 dilutions
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1 ml solute to 19 mls solvent. This gives a total volume of 20 (20 fold)
Serial dilution technique
Both force you to read your lab manual.
No, it is false. A ten-fold dilution of an acid will INCREASE the pH by 1 unit. Remember pH is 0-14 where 0 is most acidic. Diluting ten-fold on a logarithmic scale will increase the pH.Conversely, a ten-fold dilution of an alkali/base will increase the pH by 1 unit.
In ten fold dilution we add one part of the sample into the nine part of the diluent e.g. water. It will make it ten fold dilute. If we have series of tubes to dilute then after making the ten fold dilution in first tube, take the dilute sample from the first tube in same quantity as we added sample in first tube and add it to 2nd one. then then take the same quantity from 2nd one and add to third one and so on......... from the last tube we take the adjusted quantity of dilute sample and discard it. This will make the series of ten fold dilution. If you add one part substance to 10 parts of water, you get an 11-fold dilution.
1:2 dilutions
By 10-fold dilution
what is serial dilution and spread plate technique
An 8-fold dilution means you are making your solution 1/8 less concentrated. So, if you want to make 100 ml of a solution diluted 8 fold you would do as follows: 1/8=.125 100*.125= 12.5 Mix 12.5 mL of your concentrated solution with 87.5 mL of your solvent (water etc..) to bring the final volume to 100 mL. If you wanted to perform a ten fold dilution you would simply mix 10 mL of your concentrated solution with 90 mL of your solvent. 1/10=.1 100*.1=10
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Robert koch
1 ml solute to 19 mls solvent. This gives a total volume of 20 (20 fold)
Fold it 10 times....
Add one part of the substance you want to dilute to nine parts water. Nine parts water plus one part substance is 10 parts. If you add one part substance to 10 parts water, you get an 11-fold dilution.
measure 1 ml of the original solution and add 6.6 ml of distilled water.