This question is a bit general, so I am going over the possibilities. If you want to check to see if your own check will clear, you can call the bank's automated line to look up your balance. This...
At most hotels/motels, Anytime after 12pm and before 10pm check in... Check out time usually no later than 11am... but from experience you can check out at 2am that gives them time to even re-rent...
The price of checks from Checks Unlimited vary depending on the design you want. You may get a better deal if you order more than one box of checks at a time.