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How do you determine the protein-protein interaction for insoluble proteins?

In: Biology [Edit categories]

Answer:

You can't.

But consider...

1. ...that very few proteins are insoluble in their native context (that of a living organism), meaning that your attempts to mimic the conditions that the protein normally sees have failed so far. So you can try alternative conditions to make it soluble (different salts, etc).

2. ...that many proteins are composed of independently-folded modules ('domains'), and individual modules can be made and purified in isolation of the others. These isolated domains might be soluble even if the whole protein is not.

First answer by ID1170883501. Last edit by ID1170883501. Question popularity: 15 [recommend question].

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