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How do you insert a gene into a plasmid vector?
First you either find restriction sites within the gene, or add some to the ends using custom primers. Then you digest the gene with restriction enzymes specific to those sites. You can choose to...
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What is plasmid?
A segment of DNA independent of the chromosomes and capable of replication, occurring in bacteria and yeast: used in recombinant DNA procedures to transfer genetic material from one cell to another.
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What are plasmids?
plasmids are small circular strands of DNA, most commonly in a symbiotic relationship with the bacteria E. COLI.
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What is a plasmid?
A piece of DNA that is circular in shape, most common in bacteria. It codes for special characteristics of the bacteria that are not vital. A plasmid can be passed from one bacterial cell to another...
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What is the function of plasmid?
Plasmids are extra circular genetic material that can be passed from bacteria to bacteria, which basically is their function; in bacterial conjugation. But, in biotechnology it is often used in...