How do you get discrete colonies?

no, because in order to adequately study and characterize an individual bacterial species, one needs a pure culture.

it was a Proprietary and a Royal Colony. -Clitorisandra

A royal colony's governor was chosen by the British government, where as a proprietary colony governor was elected by the people in the colony who were allowed to vote.

Virgina, Georgia, South Carolina, North Carolina, Delaware, Rhode Island, New York, New Hampshire, New Jersey, Vermont, Maine, Pennsylvania, Connecticut. hope that helps