They will but there are more effective control measures for them such as addressing the areas where they roost.
Look into buying one of those rubber snakes or rubber owls (prbly at Bass Pro) that ought to scare them away
There are a few tricks: Put spikes on the surface where they are sitting; often these spikes are actually just plastic or rubber. Put a few rubber snakes - the toy kinds that look real - around...
conkers will keep away spiders apparently they dont like the smell of them, i tried this and i havent had any spiders in my home since
For a treatment you can try Mayo