Boil ¼ cup of water. Add one teaspoon of Gelatin and one teaspoon of
sugar. Stir for one minute until everything is dissolved.
2. Cool the solution for ten minutes.
3. Fill a petri dish to a little below the top edge.
4. Close the petri dish by putting on the cover immediately. Let the
covered petri dish stand for at least four hours. Don't touch the
growth medium or you'll add your own germs!
This recipe will fill two, small petri dishes. If you and your class plan to fill
more petri dishes, make more germ food by increasing the amount of your
ingredients proportionally.
Preparation of Chocolate agar: 1. 2L distilled water + 144 gm GC agar powder 2. Dissolve fully with heating till boiling 3. Autoclave 4. Cool till 45 C degree 5. Add 5% defibrinated sheep blood 6. Heat SLOWLY and evenly to 65 C degree 7. Cool till 45 C degree 8. Add IsoVitale X 1 vial, 9. Pour into plate.
http://www.umsl.edu/~microbes/pdf/homemademedia.pdf
If you add the blood to the media before it is cooled ie if the media is hot, you will make chocolate agar plates instead of blood agar plates.
collect it from seaweeds
have to incubate at low oxygen content
To make it semi-solid
Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)
Yes! Paenibacillus spp. can degrade agar agar
I teaspoon Agar Agar = 2 grams
red algae Agar agar is a malay term meaning red algae. This algae which agar is extracted lives in the ocean around Malaysia
To make it semi-solid
Agar or agar-agar is gelatin substances that can be obtain from algae. In culinary agar refers to a vegetable gelatin that is being used to make jellies, puddings, and custards products.
how to make a well on the agar for pour the extract
Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)
Agar is generally made by mixing the powdered form with varying ingredients. The powder is based on seaweed extract for nutrient agar, and nutrient agar is generally a base for most other agars - eg. Horse Blood Agar is nutrient agar with horses blood added; Choc agar has defibrinated (cooked) horses blood added.
To make the Eosin Methylene BLue Agar look cool and hip.
1) it is used by aquatic organisms as food. 2) it is to make some chemicals like agar-agar.
AGAR
Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores
Yes! Paenibacillus spp. can degrade agar agar
Agar-agar
an extract of seaweed