How do you use an eppendorf pipette?

Answer

An Epppendorf pipet is what is called a constant volume pipet. It consists of a barrel with a a push button on one end and a stem on the other. You attach a single use disposable tip to the stem. Some models allow you to set the volume to be dispensed, some are not adjustable. In use, , you depress the push button to the first stop, insert the tip into the liquid to be pipetted, and release the button smoothly to draw up the liquid. Then, transfer the pipet to the receiving vessel and depress the button past the first stop to the second stop, this dispenses the liquid and blows out the last drop to insure accuracy, then discard the tip before the next use. Every time you draw up liquid, you get the exact same volume, with very high precision.

First answer by ID2828851713. Last edit by ID2828851713. Question popularity: 13 [recommend question]