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How does the Gram stain work What is happening as each reagent is used?
Wiki User
∙ 11y ago
The positive turn purple and the negative turns red-colored. The positive is purple because the stain is able to pass through the thick peptoglycan wall where as the negative is red/pink because the stain can't get through the thick lipid layer (Membrane) to get to the thin peptoglycan layer.
Wiki User
∙ 11y ago
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How would you describe the gram stain?
Describe the Gram stain technique and the effect on Gram-positive and Gram-negative bacteria after each step. Be very specific about what is happening at each step and why it happens. (hint: be sure to fully explain your answer and not just list the steps)
List the steps of the gram-staining procedure in order and fill in the color of gram-positive cells and gram-negtive cells after each step?
____________Color of Gram-positive_______Color of Gram-negativePrimary stainCrystal violet_____Purple____________________ PurpleMordant:Iodine____________Purple________________________PurpleDecolorizing agent:Alcohol-acetone__ Purple____________________ ColorlessCounterstain:Safranin___________Purple_________________________Red
What is the specific diagnostic value of the staining procedure?
Each stain is specific for specifikc materials by example if you want to check for iron in a certain tissue and you did not stain with its specific stain you will be never able to finde it
Did you get a pure culture in your subculture and how to know?
Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
How would you describe the gram stain?
Describe the Gram stain technique and the effect on Gram-positive and Gram-negative bacteria after each step. Be very specific about what is happening at each step and why it happens. (hint: be sure to fully explain your answer and not just list the steps)
Which reactant in the reaction of sodium carbonate and calcium chloride dihydrate is the limiting reactant if 1.00 gram of each reagent is used?
calcium chloride
Which stain gives the best clarity for each organism?
Different types of organisms require different types of staining techniques. The Gram stain is majorly used in the identification of a bacterial organism. This is considered an effective stain for microscopic organisms.
List the steps of the gram-staining procedure in order and fill in the color of gram-positive cells and gram-negtive cells after each step?
____________Color of Gram-positive_______Color of Gram-negativePrimary stainCrystal violet_____Purple____________________ PurpleMordant:Iodine____________Purple________________________PurpleDecolorizing agent:Alcohol-acetone__ Purple____________________ ColorlessCounterstain:Safranin___________Purple_________________________Red
How does one determine which reagent is a limiting reagent?
To determine the limiting reagent, you must first determine moles of each reactant, and then look at the mole ratios among the reactants to see which one is in the least supply. That one will be the limiting reagent.
Why is decolorizing the most crucial step?
It is possible to decolourise gram positive bacteria and thus get false results of all gram negative organisms. Similarly, it is possible to under-decolourise and get all gram positive organisms. Ideally controls of known organisms should be run with each stain.
What is a synonym for the phrase happening each day?
Happening each day: daily.
How many tenths are in gram?
Each gram has ten tenths.
How many kilocalories are there in one gram of protein?
4 for each gram.
How many kilo calories in carbohydrates?
Each gram of Carbohydrate contains 4Kcals. Equally, each gram of Protein holds 4Kcal of energy. A gram of fat, however, contains 9Kcal of energy. Each gram of Carbohydrate contains 4Kcals. Equally, each gram of Protein holds 4Kcal of energy. A gram of fat, however, contains 9Kcal of energy.
Is 1 gram of gold the same mass as 1 gram of marshmallows?
Yes, hence - 1 gram of each.
How are sputum cultures performed?
.the sputum must be collected into a sterile container. Once in the laboratory, each culture type is handled differently. Bacterial culture.sputum is smeared on a microscope slide for a Gram stain. for mold or yeast, a fungal culture is done.
