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How does the negative stain work?

In: Microbiology [Edit categories]

Answer:

The negative staining techniques uses a dye solution in which the chromogen is acidic and carries a negative charge. (An acidic chromogen gives up a hydrogen ion, which leaves it with a negative charge.) The negative charge on the bacterial surface repels the negatively charged chromogen, so the the cell remains unstained against a colored background.

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Relevant answers:

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