Answer:
Divide your agar plate into 4 quadrants, A sterile inoculating loop is dipped ito mixed culture, streaked in a pattern over surface of nutrient medium. As pattern transferred, bacteria are rubbed off into the medium. sterilize in between quadrants, the last cells to be rubbed off the loop are far enough apart to grow into isolated colonies. Pick up a colony with sterile loop and transfer into test tube of nutrient medium to form a pure culture containing only one type of bacterium.