using uv-visible spectrophotometer
concentration vs absorbance is plotted and the maximum absorbance of the drug is lambda max of the drug. then after it will decrease. still if needed clarification, refer beer lambert"s law
The lambda max is 510 nm.
236nm
Woodward Fieser rules are a set of emperical rules to calculate lambda max. in UV spectroscopy theoretically. They can be used to calculate the wavelength of maximum absorption of dienes and conjugated carbonyl compounds.
254 nm
to ensure maximum absorbance of light by the solution
The lambda max is 510 nm.
if the protein is colorless then it is quite tough to calculate the lambda max or absorption maxima directly (still you can check it by absorbtion maxima calculation/experiment). Otherwise, for its estimation first you have to treat it with any reagent (like folin reagent) then it will form a colored complex and then you can calculate it. For the common estimation of protein, there is a "Lowry Method" where you can calculate it on 750 wavelength.
The reported UV lambda max of Vildagliptin drug is 254 nm.
257 nm
intrinsic property
495
251 NM
236nm
Woodward Fieser rules are a set of emperical rules to calculate lambda max. in UV spectroscopy theoretically. They can be used to calculate the wavelength of maximum absorption of dienes and conjugated carbonyl compounds.
520 nm
uv max of pregabalin are following: 1.in water 276nm 2.in methanol 210nm
254 nm