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How you decide the pH of mobile phase in hplc?

In: Chemistry [Edit categories]

Answer:

The pH for the individual component to be analysed, will depend on the chemical affinity of the component towards the stationary phase. The same will be different at different pH. Hence e.g. at pH 4.0 the system suitability is passing or the desired results are obtained then the pH for that analysis decided is to be 4.0

First answer by ID3398443345. Last edit by ID3398443345. Question popularity: 3 [recommend question].

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Relevant answers:

What is relationship of mobile phase pH and retention time in HPLC?
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