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What does formamide do in hybridization buffers?
Formamide and Hybridization.
Formamide lowers the melting point of nucleic acids so that the strands separate more readily. DNA is normally more stable in a double-stranded structure (even if every...
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What are the advantages and disadvantages of in situ hybridization?
The advantages of hybridisation include:
precise anatomical localisation is able to be acheived
High sensitivity
Disadvantages:
Non-specific labelling may occur
cannot provide inormation on...
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What does DMSO do in hybridization buffers?
it denatures your DNA. So you get ssDNA that can hybridize more easily.
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What buffer is?
Buffer can mean one of two things. Buffer related to the body means whoever is more in shape. If, for example, person 1 is more in shape than person 2, than person 1 is buffer than person 2. The...
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What is hybridization?
Hybridization is the crossing of dissimilar individuals to produce best of both organisms. One advantage of hybridization is that it can combine disease resistance of one organism with the production...