Sure is. How can one mate if an ecological barrier is erected between two biological species? Even something as simple as the time of day can be a barrier to mating.
Temporal isolation-species mating at different times of the year. Behavioral isolation-mating behaviors in two groups of isolated populations change, thus making individuals from the two groups not...
geographic isolation is when a physical barrier divides a population and they can no longer reproduce,
reproductive isolation is when a species can no longer reproduce offspring.
hope this helps! :)