Yes and No, either way, it doesn't matter.
If you want to write correct English then it does matter. You should separate items in a list by commas except that the last two items are separated...
This is a good question. What you are talking about is called the serial comma, or sometimes the Oxford comma. It is a comma before the last item (actually, before the "and") in a series of three or...
The use of a comma before the 'and' in a list should generally be avoided.
Its use is acceptable when it assists in the meaning of a sentence, especially where one of the items in the list is...
When "but" is used as a connective (ie. to join to sentences, with one being a negative addition to the other), it requires a preceeding comma, usefully also denoting the natural pause in speech that...