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Where are plasmids found?
Plasmids are found within the nucleus of a cell, it is a small, circular piece of DNA found in bacteria and yeasts, which is able to replicate independently of the chromosomes. They are predominantly...
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Plasmids are found only in bacteria?
Although several bacteria has plasmids, they have also been reported in certain varieties of plants and also in certain insect cells
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What are the genes on the pVIB plasmid?
It contains a gene for luciferase, a Lux gene (the enzyme that catalyzes the light-emitting reaction) and genes for enzymes which produce the luciferins (which are the substrates for the...
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Why does a gene fit into the opening in the plasmid?
When genes transfer the tra gene nicks the DNA at its origin of transfer creating a pilus so the chromosome (which contains the gene) can transfer to the other DNA.
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How do you insert a gene into a plasmid vector?
First you either find restriction sites within the gene, or add some to the ends using custom primers. Then you digest the gene with restriction enzymes specific to those sites. You can choose to...