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to differentiate between the lactose fermented bacteria or non- lactose fermented bacteria

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lactose in MacConkey agar helps to distinguish between lactose fermenters and lactose non -fermenters. it makes the medium selective for lactose fermenters.

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Q: What is the purpose of lactose in the macconkey agar?
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Does Morganella Morganii ferment lactose?

no, it shows negative for lactose fermentation on MacConkey's agar


Why does the ecoli and pseudomonas are different on the MacConkey agar?

MacConkey's agar is a differential media used to differentiate between lactose fermenting and lactose non-fermenting bacteria. E.coli is a lactose fermenter whereas Pseudomonas is a lactose non-fermenter.MacConkey's agar contains lactose as fermentable sugar and when it is fermented the pH of the medium decreases which is registered by neutral red (a pH indicator).Lactose fermenters such as E.coli produce pink colonies whereas lactose non-fermenters such as Pseudomonas produces colorless colonies. So the colors of E.coli and Pseudomonas colonies are different on MacConkey's agar.


What differences between Ecoli and pseudomonas sp on MacConkey agar?

ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.


What would be possible consequence of replacing the lactose with glucose on the MacConkey Agar?

When lactose is replaced with another type of sugar like glucose in the MacConkey Agar, it is usually to identify bacteria mutations. The method to detect the fermentation in a replacement sugar solution is the same as lactose fermented solution.


MacConkey agar inoculated with the urine specimen?

Most of the bacterial commensalism from our large intestine is lactose fermenting, Escherichia coli being one of them. When bacterial growths and colonies on MacConkey agar is pink coloured, then the organism is fermenting lactose from the medium, on the other hand, if transparent, colourless growths and colonies appear on the medium, the bacterium does not ferment lactose.The flushing action of urine, make it sterile in an uninfected healthy person. In an event where the patient is suspected to have urinary tract infection (UTI), urine of patient is inoculated on MacConkey agar medium and incubated at 37 degree centigrade overnight. In most of the cases, UTI is caused by Escherichia coli that is lactose fermenting and produces pink coloured colonies on MacConkey agar medium. Although the urine is sterile, it may get contaminated by the organisms of skin microflora or by surrounding microbes due to improper collection of urine.

Related questions

Does Morganella Morganii ferment lactose?

no, it shows negative for lactose fermentation on MacConkey's agar


How does yersinia look on nutrient agar EMB agar and MacConkey agar?

Colorless, it doesnt ferment lactose.


What is the culture characteristic of Ecoli thst grow on macconkey agar?

E.coli shows pink colour colonies in MacConkey agar due to fermentation of lactose


Why does the ecoli and pseudomonas are different on the MacConkey agar?

MacConkey's agar is a differential media used to differentiate between lactose fermenting and lactose non-fermenting bacteria. E.coli is a lactose fermenter whereas Pseudomonas is a lactose non-fermenter.MacConkey's agar contains lactose as fermentable sugar and when it is fermented the pH of the medium decreases which is registered by neutral red (a pH indicator).Lactose fermenters such as E.coli produce pink colonies whereas lactose non-fermenters such as Pseudomonas produces colorless colonies. So the colors of E.coli and Pseudomonas colonies are different on MacConkey's agar.


What is the name of the acide base color indicator found in MacConkey agar?

Lac+ bacteria is acid base color indicator that lowers the pH of the MacConkey agar. The MacConkey agar contains neutral red dye, lactose, peptone, and crystal violet dye.


What differences between Ecoli and pseudomonas sp on MacConkey agar?

ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.


What is macconkey agar made out of?

Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.


What would be possible consequence of replacing the lactose with glucose on the MacConkey Agar?

When lactose is replaced with another type of sugar like glucose in the MacConkey Agar, it is usually to identify bacteria mutations. The method to detect the fermentation in a replacement sugar solution is the same as lactose fermented solution.


Lactose fermentation mac conkey test which color indicate a positive test?

MacConkey agar is yellow (due to the lactose in the agar). When an organism consumes the lactose, the agar turns purple. Purple indicates a positive test. If the organism doesn't use the agar, the agar stays yellow.


What type of medium is macconkey agar?

types macconkey agar


Can Pseudomonas fluorescens grow on MacConkey agar?

Yes. But because it is not a lactose fermenter, the colonies will be colorless (or sometimes have a green tint).


What is the indicator that turns the agar medium pink with copper?

MacConkey agar contains bile salts, lactose, peptone. crystal violet dye, and neutral red dye. The agar appears a medium pink with a copper undertone.