there are;
1. RT PCR - helps in making complementary DNA with the help of mRNA.
2.anchored PCR - helps in making the DNA whose sequence is unknown.
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
PCR-genital testing is a specific type of test that uses polymerase chain reaction (PCR) technology to detect the presence of genetic material from pathogens in genital samples. STD testing, on the other hand, is a more general term that refers to a range of tests used to diagnose sexually transmitted infections (STIs) through various methods, including blood tests, urine tests, and swabs. PCR-genital testing is a more targeted approach that may be used as part of an STD testing regimen to detect specific pathogens in the genital area.
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
The use of dNTP is PCR and multiplex PCR
PCR stands for Polymerase Chain Reaction.
to check is there any contamination in pcr products
It Inhibits the PCR reaction by chelating the magnesium ions.
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
como reiniciar pcr 470
The choice of primers controls which DNA is amplified in PCR.
what is the difference between PCR simplex and multiplex