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What are Application of UV spectroscopy in biology?

Answer:

Nucleic Acids:

Nucleotide spectra are complicated to analyze quantitatively because there are many non-bonded electrons. Expect several different p --> p* and n --> p* transitions at each region between 200 nm and 300 nm

  1. All nucleotides have lmax near 260 nm which is not affected by sugar phos. configuration ==> can measure nucleic acids at 260 nm to estimate concentration. e260 =~ 1 x 104 M-1 ===> very sensitive and can measure concentrations down to approx. 3 µg/ml
  2. Hyperchromism -- A260 is lower for dsDNA than for ssDNA or for individual nucleotides. Results from stacking of bases in the double helical conformation; quantitative explanation is very complicated. (see Figure above)
First answer by ID3537124010. Last edit by ID3537124010. Question popularity: 4 [recommend question].

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