The Coombs' tests detect the antibodies responsible for the destruction of the red blood cells
If the test is positive, the antigens that react will narrow the search for a cause
A direct Coombs' test detects the two different antigens that might induce hemolysis in the patient's red blood cells
If the Coombs' tests are negative, the anemia is unlikely to be autoimmune, and the hematologist will have to search elsewhere for a cause
The worst complication is a bruise at the site of the puncture or punctures