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RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
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∙ 14y ago
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∙ 11y agoRFLP is a process by which purified DNA is cut into specific restriction fragments using endonucleases. We know exactly at which sequences the endonucleases cut. The DNA fragments are then put on agarose gel and run through gel electrophoresis, which separates the pieces based on molecular size. You can than visualize the fragments using special dyes or fluorescent markers (also with Southern blotting).
Because different people have insertions, deletions, and transversions in their DNA, the distances between the fragments will be different (because the sizes are different). We can use this technique to tell people apart, to establish relationships between parents and children, and to identify individuals based on DNA that has been found. RFLP is essentially used in genetic fingerprinting and paternity testing.
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∙ 15y agoRestriction Fragment Length Polymorphisms (RFLPs) Restriction Fragment Length Polymorphisms (RFLPs)
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∙ 12y agoRFLP in DNA fingerprinting stands for Restrictions, Fragments, Lengths, and Polymorphism.
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∙ 8y agoRFLP is used for DNA profiling.
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∙ 13y ago652156215
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Why is STR analysis better then RFLP analysis?
because STR only requires small pieces of DNA (2-5 base pairs long). it is fast and automated wheres RFLP can take up to a month to accomplish. STR is also better because it allows the use of the Polymerase Chain Reaction (PCR). whereas RFLP requires large amounts of non-degraded DNA and automation is not possible.
What is a person's unique RFLP pattern?
sometimes, there isn't enough DNA found at a crime scene to be properly analyzed by forensic scientists. RFLPs are used to make exact copies of the DNA sequence so that it can be better analyzed in the lab.
Can DNA sequence be cut into smaller fragments by gel electrophoresis?
electrophoresis takes segments of DNA that are already broken up and aligns them by length with an electric current. It doesn't cut the DNA.Added:No, they must be cut into smaller pieces by restriction enzymes ( HINDI, for instance ) before they are run in the gel.
Applications of chromosome walking and jumping?
You want to know the sequence of a gene and only know the sequence of 2 DNA markers near it such as an RFLP (stands for Restriction fragment length polymorph) or another gene. Using different restriction enzymes, cut the genome of the organism to create DNA clones which overlap. For example, if the genome is ABCDEFGHI, enzyme 1 cuts it into 2 pieces to make ABCD EFGHI and enzyme 2 cuts it into 3 pieces to make ABC DEF GHI.. These pieces will over lap in sequence because the enzymes have different restriction sites. Using the known sequence of the RFLP, a radio labeled nucleic acid probe can be made to search the genomic library. The probe will anneal to clones with overlaps, and the ends of these clones can be made into new probes to search for the adjacent sequence. This is repeated until the desired segment has been cloned. each clone is sequenced and the overlaps are compared to determine the order of the sequences. This technique can be used to analysis diseases, such as cystic fibrosis, to look for mutations.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
What is the difference between a gene and RFLP?
gene- meajda rflp- sjka
What can be the main limiting factor in the use of RFLP?
What can be the main limiting factor in the use of RFLP?
In molecular biology RFLP is an acronym for what?
RLFP is an acronym for Resriction Fragment Length Polymorphism. RLFP analysis is used to identify changes in a genetic sequence that occurs at a site where a restriction enzyme cuts. RFLP's can be used to identify specific mutations and also trace inheritance patterns!
What method is used to make DNA fingerprints?
A method known as RFLP (restriction fragment length polymorphism) analysis can be used to make a DNA fingerprint.
How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
What is the full form of RFLP?
Rural Functional Literacy Programmes
What is the rflps?
Do you mean "RFLP" if so its, restriction fragment length polymorphism. (DNA analysis)
Why is STR analysis better then RFLP analysis?
because STR only requires small pieces of DNA (2-5 base pairs long). it is fast and automated wheres RFLP can take up to a month to accomplish. STR is also better because it allows the use of the Polymerase Chain Reaction (PCR). whereas RFLP requires large amounts of non-degraded DNA and automation is not possible.
Is it possible for two people to have the exact same RFLP banding pattern?
It's possible, it is just very rare.
What is a person's unique RFLP pattern?
sometimes, there isn't enough DNA found at a crime scene to be properly analyzed by forensic scientists. RFLPs are used to make exact copies of the DNA sequence so that it can be better analyzed in the lab.
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
What has the author Stephen James Gray written?
Stephen James Gray has written: 'The genotyping of neisseria meningitidis by restriction fragment lengthpolymorphism (RFLP) analysis'
