Ethylene oxide and formaldehyde are two commonly used gases for sterilisation purposes
Advantages of ethylene oxide gas sterilisation
- Ethylene oxide is a gas at room temperature (boiling point 10.7°C)
- Readily permeates plastics, cardboards, cloth
- Gases are extremely effective at killing microorganisms
- The bacterial spores are only about 5 times more resistant than vegetative cells (unlike the other sterilisation methods)
- The reactive hydrogen atoms can all be replaced by hydroxyl-ethyl groups, and this replacement will interfere with a wide variety of metabolic activities
Disadvantages of ethylene oxide gas sterilisation
- Does not permeate crystals
- The levels at which it is detected exceeds safe humans for humans
- Toxicity includes burns, blistering, headache, dizziness, nausea and vomiting, weeping (lacrimation)
- Needs to be completely removed from treated products (e.g. rubber gloves) to avoid skin reactions
- Explosive mixtures are formed with air at concentrations above 3%
- Humidity may decrease activity
Advantages of formaldehyde gas sterilisation
- Formaldehyde is a gas at room temperature (boiling point - 19°C)
- Very reactive molecule, with a small difference in effectiveness between spores and cells (as is the case with ethylene oxide)
- Acts as a mutagenic agent, reacting with carbonyl, thiol, and hydroxyl groups
Disadvantages of formaldehyde gas sterilisation
- The vapour is extremely irritating to the eyes
- Weak penetrating power, and so is only really a surface bactericide
- The gas is difficult to remove and requires long airing times
- A relative humidity of ~ 75% is required in order to be effective as the gas has to dissolve in a film of moisture surrounding the bacteria
This is a procedure of sterilization using a gas.
This is a procedure of sterilization using a gas.
The gas commonly used in gas sterilization is ethylene oxide (EtO).
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cold sterilization can and is done in a soak of Cidex solution (glutaraldehyde) in the medical field. Sterilization can be done with steam, gas, and solutions like Cidex.
EtOH which is ethylene gas. You could opt for vaporized hydrogen peroxide. Both are hazardous to use and often less effective than steam sterilization.
Yes. Chemical sterilization and cold sterilization are the same thing.
Fiona
it gives me gas
Advantages: 1. It is faster method of sterilization then dry heat method. 2. Additional parameter "pressure" is involved 3. We can easily sterilize the media solution. 4. It faster method of sterilization. Disadvantages: 1. Glass ware might get broken down. 2. Mishandling may lead to serious accident or explosion.
To much gas
A Bunsen burner is a of laboratory equipment that produces a single open gas flame, which is used for heating, sterilization, and combustion. The gas can be natural gas (which is mainly methane) or a liquefied petroleum gas, such as propane, butane, or a mixture of both.