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Basic dyes:
Crystal violet, Methylene blue, Malachite green, Safranin.
Acidic dyes:
Nigrosin, Congo red
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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Can you stain on top of the old stain?
yes you can and the stain can change colors if there is a juice stain for example thats red and a grass stain the stain color could change
Predict the acidic basic or neutral character of the solutions of the following salt?
acidic character
How do you remove the green stain on metal?
You can remove the green stain on metal by treating the rust stains with a cleaning agent such as a lime remover or some kind of harsh acidic substance.
Which of the following is most likely to not acidic?
blood
What one of these leucocytes stain both acidic and bacic dyes?
Neutrophils stain both acidic and basic dyes
What is the pH of a carbolfuchsin direct stain?
Acidic
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
What are the differemt kind of stain?
Acidic,basic and neutral stains
Is counterstain a negative stain?
Yes. Eosin and acid fuchsin are also acidic dyes like nigrosin.
Can you stain on top of the old stain?
yes you can and the stain can change colors if there is a juice stain for example thats red and a grass stain the stain color could change
What the principle of leishman stain?
It is basically use to stain leukocytes,maleria prasite and trypanosomas. leisman stain contain 1st methylene blue dye, a basic dye, which gives color to an acidic component.2nd eosin dye,an acidic dye ,which gives color to a basic component. These dye differentiat the different component of blood.
What is the principle of leishman stain?
It is basically use to stain leukocytes,maleria prasite and trypanosomas. leisman stain contain 1st methylene blue dye, a basic dye, which gives color to an acidic component.2nd eosin dye,an acidic dye ,which gives color to a basic component. These dye differentiat the different component of blood.
Why is size more accurate in an acidic or negative stain than in a simple or basic stain?
Since there is no heat fixing or strong cemicals are used the bacteria are less distorted
Predict the acidic basic or neutral character of the solutions of the following salt?
acidic character
Why an acidic stain is used in negative staining?
Acidic dyes are negatively-charged dyes. Since bacteria are also negatively-charged, they will repel the acidic dyes. So, instead of staining the bacterium itself, it will be the background that will be colorized....
What is stain on shirt an example of?
it is an example ostrawberry jam
