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What are the reactions involved in Lieberman Buchard test?

Answer:

When conc. sulfuric acid is added to cholesterol, a water molecule is removed from C3 of cholesterol molecule, and it is oxidised to form3,5-cholestadiene.This product is converted to a polymer containing a chromophore which results in the green colouration indicating a positive test.

First answer by ID3397172717. Last edit by ID2083207171. Question popularity: 1 [recommend question].

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