I dont have any answer, But i will post it when i will know . No change in color or reaction on Indole Test - Negative result on Indole Test.
It does not have a capsule
a huge stain. a jumbo stain. a large stain.
Bleach will take a stain out.
Gram Negative
Wright's Stain is a mixture of methylene blue and eosin in methanol. Gram's stain is crystal violet, iodine washed with acetone and proofed with a safranin dye to look for gram negative organism.
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
Gram staining protozoans yield variable results. Endospore, capsule, and AF stains will yield different results, as these stains are use on bacteria. Malaria is caused by a protozoan.
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
to stain the outside and the inside of the cell.. so the capsule appears transparent
yes as it have capsule it give positive test for capsule staining when stain with malachite green The problem with the above answer is that a capsule stain is NOT done with malachite green.
To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
Capsule Stain
capsule
capsule
Demonstrating the presence of a capsule is a means of diagnosis and determining the organisms virulence.
The copper sulfate is used as a decolorizing agent rather than water. The copper sulfate washes the purple primary stain out of the capsular material without removing the stain bound to the cell wall. At the same time, the decolorized capsule absorbs the copper sulfate, and the capsule will now appear white in contrast to the deep purple of the cell.
From what i read in my book: Because the capsule is nonionic, unlike the bacterial cell, the primary stain adheres to the capsule without binding to it. Since the capsule is water- soulube, copper sulfate, rather than water, is used to wash the purple primary stain out of the capsular material without removing the stain that is bound to the cell wall.