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Answer:
The Hanging Drop Procedure
The direct examination of live microorganisms can be extremely useful in determining size, and shape relationships, motility, and reactions to various chemicals or immune sera. Two methods are generally used for this type of study;hanging drop and wet mount. Both methods maintain the natural shape of microorganisms and reduce the distorted effects that can occur when specimens are dried and fixed. Because the majority of microorganisms are not too different in either color or refractive index from the fluid in which they are suspended, low light intensity is suggested (closed iris).
Purpose: To observe live culture motility using the Hanging Drop method.
To know the difference between true motility (vital movement) and
Brownian motion.
Materials: 24 hour (37oC) nutrient broth culture of Bacillus subtilis
3 day old culture of Saccharomyces cerevisiae ( yeast suspension)
Microscope slides (concave), coverslips, washers (spacers)
Inoculating loop and Inoculating needle, Immersion oil
The direct examination of live microorganisms can be extremely useful in determining size, and shape relationships, motility, and reactions to various chemicals or immune sera. Two methods are generally used for this type of study;
To know the difference between true motility (vital movement) and
Brownian motion.
Materials: 24 hour (37oC) nutrient broth culture of Bacillus subtilis
3 day old culture of Saccharomyces cerevisiae ( yeast suspension)
Microscope slides (concave), coverslips, washers (spacers)
Inoculating loop and Inoculating needle, Immersion oil
Procedure:_Hanging _Drop_See_Diagram_6">Procedure: Hanging Drop See Diagram 6
- Aseptically transfer a loop full of yeast (Saccharomyces) suspension to the center of a new glass coverslip.
- Aseptically transfer a loop full of the Bacillus culture to the same glass coverslip. Together, they should make a small
- Obtain a concave slide and place it face-up on the table in front of you. Place a yellow washer on the slide so that it surrounds the depression in the slide.
- Carefully flip the coverslip containing the microorganisms over and place it on top of the washer being careful not to let the culture
- Observe the edge of the
- Try to observe both bacteria and yeast. Look for motility (vital movement) as well as Brownian motion.
- aashi....
First answer by ID3673702900.
Last edit by ID3673702900.
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