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What causes a dark band on the chromosome?

Answer:

When DNA coils into a chromosome, it keeps its shape with the help of associated proteins. Where the DNA is very tightly coiled, and the proteins very condensed, that region appears as a dark band (G-band). G-bands are not very rich in genes, which means that it's ok that the DNA is tightly coiled, because it does not have to be very accessible anyway. Note that these bands are only visible when certain types of dye are used. Other types of dye will case gene-rich regions to stain darker and gene-poor regions to stain more brightly.

See also: Chromosomes: Organization and Function By Adrian Thomas Sumner, 10.2.2

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