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Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.

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Q: What would you observe if you forgot to use oil with the oil immersion lens?
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Related questions

When would you need to use the oil immersion lens?

Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.


What mirror is used for oil immersion?

100x lens is used with oil immersion


Oil immersion is used as a medium between the specimen and the oil immersion objective lens why?

Oil immersion is a technique used to increase the resolution of a microscope.


Should you store the microscope with the oil immersion lens in position?

no


How immersion oil helps in viewing an object?

This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.


Why is 100x microscope lens called oil immersion lens?

Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.


Why do you use on or slide to be examined with the oil immersion objective?

If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.


Which objective focuses closest to the slide when it is in focus?

The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.


Is the low power objective lens the shortest objective lens?

scanning objective


What objective focuses closest to slide?

The oil immersion lens @100x


Does the microscope get stored with oil immersion lens in position over the stage?

no


What tool would you use to observe the luster of a rock?

A magnifying lens would be handy for observing luster.