Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
scanning objective
The oil immersion lens @100x
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
100x lens is used with oil immersion
Oil immersion is a technique used to increase the resolution of a microscope.
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This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
scanning objective
The oil immersion lens @100x
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A magnifying lens would be handy for observing luster.