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Both bacteria types would be stained by the safranin. When the iodine is added, safranin would be "set" in the positive. The decolorizer would wash out the safranin and then application of the crystal violet would stain the negative.

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13y ago
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9y ago

During gram staining, if in the application process crystal violet was reversed with safranin by accident. The safranin would be washed out and/or covered up by the darker crystal violet dye.

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11y ago

Then, there might be an inaccurate result or might get wrong in reading the possible bacteria present in the specimen.

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9y ago

Gram positive bacteria will remain violet, but without the counterstain gram negative bacteria will not be the recognizable pink/red color they need to be in order to classify the organsim.

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12y ago

gram-negative bacteria will be clear or colorless

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Q: What happens if not enough alcohol is applied to a gram stain?
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