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Taq are important in PCR because they are heat resistant and are required for polymerase action. Hence we use Taq Polymerase enzyme in PCR. Specially and their main role is "Heat Sensitive Polymerase Enzyme".
Wiki User
∙ 14y ago
Wiki User
∙ 14y agoPCR is an abbreviation for Polymerase Chain Reaction. The polymerase chain reaction amplifys the amount of DNA you have in a sample. It does this by repeatedly heating and cooling the DNA. Heating separates the strands and cooling allows the creation a complimentary strand. The technology only became avalible with the discovery of heat stable DNA polymerases such as those found in the themophilic bacteria Thermophilus aquaticus hence the commoly used DNA polymerase "Taq".
Wiki User
∙ 11y agoIt is the mechanical and automatic way of DNA amplification. In various biotechnological experiment moderate quantity of DNA is required which can be obtained from small extracted DNA by using PCR. Even site specific mutation can be induced during PCR. There are certain PCR based DNA fingerprinting techniques are available.
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Why do you use a negative control in PCR?
to check is there any contamination in pcr products
What is the function of EDTA in PCR?
It Inhibits the PCR reaction by chelating the magnesium ions.
What is the defference between Real-time PCR and reverse transcriptase PCR?
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
What controls which DNA is amplified in PCR?
The choice of primers controls which DNA is amplified in PCR.
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
PCR stands for?
PCR stands for Polymerase Chain Reaction.
Why do you use a negative control in PCR?
to check is there any contamination in pcr products
What is the function of EDTA in PCR?
It Inhibits the PCR reaction by chelating the magnesium ions.
What is the defference between Real-time PCR and reverse transcriptase PCR?
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
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What controls which DNA is amplified in PCR?
The choice of primers controls which DNA is amplified in PCR.
What is the difference between simplex and multiplex pcr?
what is the difference between PCR simplex and multiplex
Which is used to copy DNA for DNA fingerprinting?
PCR
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
