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What does Rf Values in chromatography represent?

Updated: 8/10/2023
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WilliamTang

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14y ago

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It is the rate of flow for each color. It is calculated by taking the value for the measured from the initial color mark to the solvent front (value A) and then the distance from the initial spot to each of the centers of the color marks separated (value B). You then divide B by A.

For example, solvent front for black felt tip pen is 5.4cm and the distance for value B is .9 cm. The rf for this problem is .9cm/5.4 cm = .17 (Rf factor is a unitless number)

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14y ago
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14y ago

Rf is the relationship of the distance moved by a pigment to the distance moved by the solvent.

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15y ago

Rf value is the ratio of distance travelled by the solute to the distance travelled by the solvent front.

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12y ago

Rf has no units because it is the ratio of two distances, the distance travelled by the sample divided by the distance travelled by the solvent.

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11y ago

Rf value is the ratio of distance travelled by the solute to the distance travelled by the solvent front.

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13y ago

retardation factor also know as sometimes retention factor

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12y ago

Retention Value, is also known as Rf value.

Retention Value, Rf can be defined as the ratio of distance moved by the solute and the distance moved by the solvent.

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7y ago

Rf is the distance traveled by the substance of interest divided by the distance traveled by the solvent front. It stands for "retention factor".

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15y ago

RF=Retardation Factor Ratio of Fronts

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14y ago

Stands for reference front (Rf)

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Q: What does Rf Values in chromatography represent?
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What is the significance of Rf value in chromatography?

See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.


What are the variables in a plan and design paper chromatography lab?

responding variable-distance of components( Rf values)


How will you find the Rf values of green tea components. Rf values have been calculated but don't know what the represent. Can you help?

well first u do the whole chromatography thing. then u divide the solvent(distance the water traveled) by the distance the green tea components traveled alone. Then u should get you answer and should be between 0-1. Have a nice day;)


Would RF values of amino acid you use in paper chromatography be the same if you use another solvent system?

Rf value is the ratio of distanced covered by solute by distanced covered by solvent.


How is rf factor in chromatography calculated?

It's not.


Difference Rf values for monosaccarides and disaccharides?

Monosaccarides contain fewer polar covalent glycosidic bonds than polysaccharides and therefore move further up during thin layer chromatography and column chromatography.


What are the significance of Retention factor values?

Rf Values determine the solubility of a substance with respect to a certain solvent. It also determines the affinity of the solute to the solvent (greater Rf=greater affinity of solute to the solvent)


How do you calculate Rf in chromatography?

In chromatography, Rf is the distance the solute travels divided by the distance the mobile phase travels. For example, in thin layer chromatography, if the spot travels 7 cm, and the mobile phase travels 15 cm, the Rf value for that spot will be 7/15 = 0.47


How do you measure Rf?

Rf value = distance traveled by a compound/distance traveled by a constant.Source: http://www.chemguide.co.uk/analysis/chromatography/paper.html


What is an rf value used for?

The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.


How would Rf values changes of Cations if the developing chamber left uncovered in paper Chromatography?

The solvent would keep on evaporating from the chromatogram and as a result it would keep on bringing the cations up, causing an increase in the Rf value.


How you might use Rf values to identify an unknown substance?

Although Rf values technically remain constant if the following are held constant: * solvent system * adsorbent * thickness of the adsorbent * amount of material spotted * temperature They are generally not used to identify an unknown substance specifically, since these factors are sometimes difficult to control. Most generally, during a chromatography experiment an unknown sample can be compared directly to a known sample simultaneously, i.e. they will have similar Rf values.