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It is a procedure to stain the bacterial capsule to study its presence and also morphology for this the following steps are performed:
Place a drop of Congo red on one end of the slide
Aseptically pick up a sample from a culture with your loop and mix briefly with the Congo red stain.
Spread the mixture across the slide
Air dry ONLY. Proceed to inoculate your tubes while drying.
Stain 1-2 minutes with the A-M Capsule Stain Solution. S(THIS STAIN CONTAINS PHENOL)!
Rinse very gently, air dry,
Look for red cells surrounded by a clear, colorless zone on a blue background.
Wiki User
∙ 14y ago
Wiki User
∙ 12y ago1. Place a few drops of Congo red on a clean slide. Do NOT use a water drop in this sample preparation.
2. Mix in a small amount of culture.
3. AIR DRY. DO NOT HEAT FIX. (Heat fixing destroys protein capsules; it could also dehydrate the cells which would result in shrinking of the capsule)
4. Place the slide on a rack of the staining tray. Gently flood the smear with the Maneval solution and wait 5 minutes.
5. Lift the slide and gently pour off excess stain.
Rinsing with water is the next step. Rinsing must be done with great care. As the sample was not heat fixed to the slide, the sample preparation is fragile. Suggestions for careful rinsing:
· Place slide on bottom of staining tray.
· Add water to tray in the opposite corner from the placement of the slide. Add the water SLOWLY
until a large puddle forms.
· Tilt staining tray and allow water to rinse the slide. Remove slide from tray.
6. Place slide on absorbent paper. Allow sample to AIR DRY. Do not blot.
7. Examine the slide with an oil immersion lens at 1,000X magnification.
Wiki User
∙ 14y agoklebsiella pneumoniae,Bacillus sps,Enterobacter aerogenes etc. are encapsulated bacteria which comprises poly saccharides that prevents bacteria from digestion by certain enzymes.
Wiki User
∙ 13y agoCapsules are not common to all organisms. Cells that have heavy capsules are generally those capable of producing disease.
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What is the appearance of capsule after being stain with capsule stain procedure?
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
What are the materials used in capsule stain?
For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
What is the other name for Negative staining?
Capsule Stain
A stain that does not penetrate cell use to color background?
capsule
How is the capsule stain used in clinical microbiology?
Demonstrating the presence of a capsule is a means of diagnosis and determining the organisms virulence.
What is the appearance of capsule after being stain with capsule stain procedure?
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
What are the materials used in capsule stain?
For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
What is the purpose of emulsifying the bacteria in the capsule stain?
to stain the outside and the inside of the cell.. so the capsule appears transparent
What is the other name for Negative staining?
Capsule Stain
What is the slime layer that surround gram negative bacteria and keeps them from taking up purple stain?
capsule
A stain that does not penetrate cell use to color background?
capsule
How is the capsule stain used in clinical microbiology?
Demonstrating the presence of a capsule is a means of diagnosis and determining the organisms virulence.
What is the function of copper sulfate in the capsule stain?
The copper sulfate is used as a decolorizing agent rather than water. The copper sulfate washes the purple primary stain out of the capsular material without removing the stain bound to the cell wall. At the same time, the decolorized capsule absorbs the copper sulfate, and the capsule will now appear white in contrast to the deep purple of the cell.
Explain the function of Copper Sulfate in the spore staining procedure?
From what i read in my book: Because the capsule is nonionic, unlike the bacterial cell, the primary stain adheres to the capsule without binding to it. Since the capsule is water- soulube, copper sulfate, rather than water, is used to wash the purple primary stain out of the capsular material without removing the stain that is bound to the cell wall.
Does a micrococcus luteus is positive for capsule stain?
yes as it have capsule it give positive test for capsule staining when stain with malachite green The problem with the above answer is that a capsule stain is NOT done with malachite green.
What is the slime layer gram negative bacteria and keeps them from taking up purple stain?
capsule
What is the color of the capsule in the capsule stain?
When a stain, such as an acid dye, cannot penetrate the outer layers of a microbe, the cell will appear transparent on a colored background. This stain is called a negative or background stain. It is performed by mixing the dye with a suspension of bacteria on a slide and spreading the mixture into a thin layer for viewing. The capsule is a structure surrounding the cell wall that certain bacteria can produce. The ability to form a capsule is genetically and environmentally controlled. Only those microbes with the genes for capsule production have the potential to manufacture this polysaccharide (or polypeptide) surface layer. Special nutrients or other growth factors often are necessary for the genes to be expressed. The role of the capsule is primarily for protection of the bacteria. For example, the capsule affords a seal against dehydration. Many capsules repel white blood cells and thus allow pathogenic invading bacteria to elude one of the primary host defenses. Capsules are not readily stained and therefore are visualized by negative stain techniques. The organisms are prepared as a smear in the presence of an acid dye and allowed to air dry because heat will cause the capsule to shrink. Our procedure will combine a negative stain (which colors the background) and a simple stain to color the bacterial cell. The capsule appears as a colorless layer between the bacterium and the background.
