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What is a polar column?

Updated: 12/16/2022
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Q: What is a polar column?
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If the column is polar then what types of illution first polar or non-polar?

if the column is polar then non polar ellute first because of its less interaction with that of stationary phase.


Can you use silica in place of Alumina?

assuming your talking about column chromatography. Yes, they are just two different absorbents that are polar to slow the polar eluate as it runs down the column.


Are polar bears invertebrates?

No. They actually do have a backbone and a spinal column.


How do you select hplc column in method development?

firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column. after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud


How is the movement of acetylferrocene during the addition of hexane to the column?

The acetylferrocene barely moves because it is attracted to more polar substances and hexane is a less polar substance.


Is a polar bear a Chordata?

Yes. Any creature with a spinal column and vertebra are of the Phylum Chordata.


what is the difference c3 and c4 hplc column?

The difference is the length of the carbon alkyl chain off of the silica bead support in the column. The higher the number, the longer the chain, the more "reverse phase" it is. For example a polar compound is best separated on a C3 if you must use reverse phase and a non-polar compound will be best separated on a C18 column.


In Normal Phase HPLC which elutes first n-hexane or benzene Both are non-polar so the retention time on the column should be low but I don't know which one is most nonpolar.?

You can look up solvent polarity by looking up their dielectric constant (the higher the constant the more polar it is). n-hexane has a dielectric constant of 2.0, where as benzene has a dielectric constant of 2.3. This means that benzene is more polar than n-hexane. (Source: http://en.wikipedia.org/wiki/Solvent). On a Normal Phase HPLC column, the column is coated in silica gel which is very polar. The rule "like dissolves like" is important, as you pass your sample through the column the polar molecules will grip on tighter to the silica gel, where as the non-polar molecules will flow through easier. This means that in a Normal Phase HPLC "race" down the column, it is the n-hexane that will come out first.


What is role of ion pair reagent in mobile phase of HPLC?

The reagent couples with the analyt, making the analyt polar. Now, the reagent-analyt complex can interact with the polar column.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


How does hplc works?

HPLC works when a reservoir holds the solvent and then it is sent to the pump manager.Next it goes to the HPLC coloumn .After it goes through there it usually ends in the detector than waste. Generally the stationary phase in the HPLC column is made up of alkyl coated silica making it relatively non-polar. Due to this the technique is also called reversed-phase HPLC.


What would have been the result if a large quantity of petroleum ether alone were used as the eluent in either of the experiments described in column chromatography?

petroleum ether is a lot less polar than solvents like MTBE and the hexanes. so if the stationary phase is a lot more polar than the solvent then the components of the mixture that were added to the column to be separated will get stuck in the stationary phase