What is a vitro study?

A Vitro study is anything to do with a basic cell function outside the body. To explain in Vitro systems the secretory pathway is used.
To use cells contents, membrane fractionation of organelles is used. This isolates them and you can see what proteins you are dealing with. George Palade suggested that the secretory pathway controls movement from the ER to the Golgi.

What do you need to design your own Vitro study?

First you need sufficient components to support the process of interest.
A process that you want to study
An essay that will determine this

Lets go over the production of a key Vitro process production of COPII vesicles.

NSF is a protein required for fusion.. Not vesicle formation. NSF works on the GTP-NSP to not hydrolyze the ARF1 protein.

Arf is relent on COP to form vesicles.

This is how a vitro study is performed and is analogous to In vitro fertilization study. The researchers will take the egg and sperm and study the cellular components the cell lines.