What are the limitations of Beer's Lamberts law?

Answer 1

The linearity of the Beer-Lambert law is limited by chemical and instrumental factors. Causes of nonlinearity include:

• deviations in absorptivity coefficients at high concentrations (>0.01M) due to electrostatic interactions between molecules in close proximity
• scattering of light due to particulates in the sample
• fluorescence or phosphorescence of the sample
• changes in refractive index at high analyte concentration
• shifts in chemical equilibrium as a function of concentration
• non-monochromatic radiation, deviations can be minimized by using a relatively flat part of the absorption spectrum such as the maximum of an absorption band
• stray light

Answer 2

Beer's Law, also known as the Beer-Lambert law, relates the attenuation, or reduction, of light to the properties of the material it passes through. It's mainly related to the BGK model, which is a mathematical model that helps describe collisions of particles.

Answer 3

It is used to measure the concentration of a substance in a solution by making use of the absorbance of electro magnetic radiation by the substance.It is applied widely in the field of bio chemistry - specifically in protein analysis of research and medical samples

Answer 4

The Beer-Lambert Law, also known as Beer's law, is a law of spectrophotometry that states the relationship between how a sample absorbs light and the quantity of a known substance within the sample. This is highly useful in many fields of medical testing, where it is applied to determine, among other things, the concentration of a chemical or substance in blood by analyzing how the blood sample absorbs light.

Answer 5

Analytical determination by absorption spectrophotometry are based on the Lamber-Beer law.

Answer 6

The law is not valid at high concentration; more details at this link.