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Beer and Lambert's law states that 'when a ray of monochromatic light passes through a solution in a transparent vessel, intensity of transmitted light depends on concentration of absorbing solution and path length of absorbing medium.'
A=ebc
Where A is absorbance (no units, since A = log10P0 / P )
e is the molar absorbtivity with units of L mol-1 cm-1
b is the path length of the sample - that is, the path length of the cuvette in which the sample is contained. We will express this measurement in centimetres.
c is the concentration of the compound in solution, expressed in mol L-1
when path length is kept constant ansorbance is proportional to concentration of substance. Path length for a colorimeter is constant.
Different substances absorb light of different frequencies maximally. Light of appropriate frequency is passed through solution using different filters in colorimeter and % transmission is measured. e.g. proteins absorb light of 650nm.
Error is eliminated by calliberatin blank(solution without test substance) as 100% transmission ie zero absorbance, and black stop as 0% transmission. Thus we get absorbance of only required substance.
% transmission is converted to absorbance using following expression
A = 2 - log10 %T
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∙ 12y ago
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∙ 13y agoA spectrophotometer is used to measure the amount of light a sample (be it liquid or solid) absorbs. Light is passed through the sample and a detector measures the intensity of that light that passes through the sample and hits that detector.
The beam of light consists of photons, and when these photons encounter an analyte molecule (an analyte being the molecule that is under study), there's a chance that this analyte will absorb the photon. This absorption consequently reduces the number of photons in the light beam and thus reduces the intensity of that light beam.
See related link below for a demo.
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∙ 12y agoThe science of measuring colours and assigning them numeric values; Analysis involving the quantitative measurement of colour and is done especially by big Dave McC
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∙ 15y agoThis is an analytical technique which depends on the measurement of colour intensity of solutions.
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∙ 11y agocoiorimetry is an analytical procedure whichdeals with the abrorption of radiation
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∙ 9y agoA spectrophotomer is an apparatus which is designed to measure the intensity of light in a spectrum. Generally, a spectrophometer is used in spectral regions near the visible spectrum.
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∙ 12y agoused in food industries and other various companies to test condoms
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∙ 12y agoBy colour base
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What is the difference between colorimeter and turbidimeter?
What is technical difference between colorimeter and turbiditymeter
How does a Colorimeter work in Colorimetry?
A Colorimeter can measure the absorbency of light waves. The Colorimeter has a set of changeable filters that can show which color of light to examine for greatest accuracy.
What is cascading principle?
If you mean the cascade principle; that is a principle connected to evolution in Cellbiology.
What rule or principle states that no two electrons in the same orbital can have the same spin?
This is the Pauli exclusion principle. Wolfgang Pauli was a Jewish physicist, Nobel prize laureate.
What do you mean by SHAB principle?
what is shab principle
What is colorimeter reading?
the reading on a colorimeter, abit obvious :S
What is the difference between colorimeter and turbidimeter?
What is technical difference between colorimeter and turbiditymeter
What is meant by photoelectric colorimeter?
Photoelectric colorimeter is a medical term. Essentially, it is referring to a colorimeter using a photoelectric cell and appropriate filters instead of the eye.
How does a Colorimeter work in Colorimetry?
A Colorimeter can measure the absorbency of light waves. The Colorimeter has a set of changeable filters that can show which color of light to examine for greatest accuracy.
How could a colorimeter be used in a breathalyzer test?
A colorimeter is used to measure the intensity of color. The colorimeter would detect an intensity of a blue green color. When alcohol that is present increases.
How do you use a colorimeter?
we can use colorimeter for quantitative analysis of a specific substance which absorb light in the visible region.
How does colorimeter work?
By colour base
Who discovered the colorimeter?
It is not something that was discovered, it was invented. One of the most popular designs is the Duboscq colorimeter which was invented by Jules Duboscq in 1870.
How would you calibrate a colorimeter?
A colorimeter is simply a sensor of color. The instrument is normally labelled clearly for easy use by new users.
How many filters are present in colorimeter?
Typically a colorimeter has three filters. However, this can change depending on the company and type of colorimeter. For example our Gamma Scientific tri-stimulus colorimeter uses four extremely stable colored glass filters in conjunction with high quality silicon photodiodes for increased accuracy. This is done to more accurately match the CIE standard observer functions. These resources may be helpful if you have further colorimeter questions:
What is the principle behind the working of colorimeter?
the colorimeter is an instrument to measure absorbance of light. wen u select the range 600 pr 540nm or any, it cutts of al othr light n allows nly light of tat range to pass thru it and the digital meter gives the transmittence. by plotting the graph one can estimate the amount of sample present in the solution.
Why you are using blank in colorimeter?
we are using blank because if we are not inserting anything in colorimeter and keeping it open then the light from the surrounding may affect it's absorbance causing damage
