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Answer:
Biurets test is used to identify if there are proteins present in a substance.
The bond between the amino group and the carboxyl acid group on adjacent amino acids in a protein is a peptide bond.
When the Biuret reagent (1 percent solution of copper sulfate) is added to a solution containing peptide bonds, the solution turns a violet color. The violet color is a positive test for the presence of protein. The more intense the color, the greater the number of peptide bonds that react.
hence, if it turned purple then there are proteins
but if it stayed blue then no proteins are present .... mostly it is used to check for enzymes which are made of proteins :D improved by hala saad <3
The bond between the amino group and the carboxyl acid group on adjacent amino acids in a protein is a peptide bond.
When the Biuret reagent (1 percent solution of copper sulfate) is added to a solution containing peptide bonds, the solution turns a violet color. The violet color is a positive test for the presence of protein. The more intense the color, the greater the number of peptide bonds that react.
hence, if it turned purple then there are proteins
but if it stayed blue then no proteins are present .... mostly it is used to check for enzymes which are made of proteins :D improved by hala saad <3
Contributor:
Halool
Halool
First answer by Halool Saad.
Last edit by Halool Saad.
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