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What is the chemical composition of fluid thioglycollate medium?
Wiki User
∙ 10y ago
Enzymatic Digest of Casein 15 g,Yeast Extract 5 g,Dextrose 5.5 g, L-Cystine 0.5 g,Sodium Chloride
2.5 g,Sodium Thioglycollate 0.5 g,Resazurin 0.001 g, Agar 0.75 g
Final pH: 7.1 ± 0.2 at 25°C
Formula may be
adjusted and/or supplemented as required to meet performance specifications
Wiki User
∙ 10y ago
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liquid or water
What does a blue or pink color in thioglycollate mean?
If its Fluid thioglycollate media - a red colour indicates thatg the media has been oxygenated - which is not desirable. The colour change is due to a chemical called resazurin in the media.
Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
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chemical composition mean it is made of body fluid.
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
What is the chemical composition of skeletal muscle?
The chemical composition of skeletal muscle is 75% H2O, 20% protein, 5% other ( such as, electrolytes like Na+,Ca++, also included in other is Fat and glucagon
What is the medium for all body fluids in the body?
Interstitial fluid is the basic fluid from which all others are derived, including blood, lymph, and all extracellular fluid. Hormones are chemical messengers and are transported by blood, thought they themselves are not considered a body fluid.
Different between GM ford power steering fluid?
The difference between GM and Ford power steering fluid is in their chemical composition. Both have unique blends designed to work best in their equipment.
Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?
The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.
What is the composition of the dialysis fluid?
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What is the importance and function of cytosol to the brain?
Cytosol is the fluid found inside cells and is the medium where cellular metabolism takes place. It has a slightly different composition to the fluid found outsides cells (extracellular fluid). In neurons, this difference in fluid composition is critical for cell signaling because it creates an electrochemical potential gradient across the cell membrane. When ions flux in and out of the cell (driven by chemical gradients and 'ion pumps') their charge alters the electrical potential difference across the cell membrane (i.e., think of the membrane as an insulator separating positive and negative charges). If enough positive charge enters a neuron then it will fire (release it's neurotransmitters).
Plasma is closest in composition to?
interstitial fluid.
