Antimicrobial Agents and Chemotherapy, January 2010, p. 509-512, Vol. 54, No. 1
0066-4804/10/$12.00+0 doi:10.1128/AAC.00940-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Method for Screening Compounds That Influence Virulence Gene Expression in Staphylococcus aureusAnita Nielsen,1 Kristian F. Nielsen,2 Dorte Frees,1 Thomas O. Larsen,2, and Hanne Ingmer1*Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, DK-1870 Frederiksberg, Denmark,1 Department of Systems Biology, Technical University of Denmark, DK-2800 Lyngby, Denmark2
Received 8 July 2009/ Returned for modification 3 August 2009/ Accepted 2 November 2009
We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agrlocus are scored based on color change in the presence of a chromogenic β-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
* Corresponding author. Mailing address: Department of Veterinary Disease Biology, Stigboejlen 4, 1870 Frederiksberg C, Denmark. Phone: 45-3533-2773. Fax: 45-3528-2757. E-mail: hi@life.ku.dk
Published ahead of print on 16 November 2009.
Antimicrobial Agents and Chemotherapy, January 2010, p. 509-512, Vol. 54, No. 1
0066-4804/10/$12.00+0 doi:10.1128/AAC.00940-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
http://aac.asm.org/cgi/content/abstract/54/1/509?maxtoshow=&hits=10&RESULTFORMAT=&fulltext=staphyloccocus&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT
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The conflict in the story of The Aged Mother is... A despotic leader proclaimed a cruel order to immediately put to death all aged people....
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In the story "The Aged Mother," the dénouement is when the decree to sacrifice all the elderly is lifted, the Emperor realizes the importance of his mother, and the mother and son are reunited. It serves as the resolution of the conflict and brings closure to the story.
im asking question 2
character, setting, plot, theme, conflict
The farmer is ordered to abandon his beloved mother to die because she is old and weak.
The farmer is ordered to abandon his beloved mother to die because she is old and weak.
the story of the aged mother
the story of the aged mother
the story of the aged mother
where the story happen the aged mother